Description

Soluble Transferrin Receptor or sTfR ELISA Test kit




Soluble Transferrin Receptor (sTfR) ELISA Test kit Intended Use
The sTfR ELISA Kit is for the quantitative determination of sTfR concentration in human serum or plasma by a microplate enzyme immunoassay.




sTfR ELISA Kit Components:
• Microplate: 96 wells coated with Streptavidin
• sTfR Calibrators
• sTfR-HRP Conjugate
• sTfR Biotin Reagent
• Wash Solution Concentrate
• Substrate Solution
• Stop Solution




Method Principle
This Diagnostic Automation/ Cortez Diagnostics Inc. Soluble Transferrin Receptor or sTfR ELISA Kit is a quantitative test based on the principle of Immunoenzymometric sequential assay. For further details please refer to complete instructions manual supplied with the product.




Background Information
sTfR measurement provides insight into diagnosis of many diseases and offers a new diagnostic tool. A key step into differentiating between iron deficiency anemia (IDA) and anemia of chronic disease (ACD) is sTfR level in blood which determins whether iron supplementation would be beneficial. sTfR is also useful for monitoring erythropoiesis in malignancy and chronic renal disease. Development of erythropoiesis following bone marrow or stem cell transplantation is determined by the overall marrow proliferative capacity, which can be monitored with sTfR. During the aplastic period prior to transplantation, sTfR levels decline. Once erythropoiesis has recovered, sTfR levels return to normal values. In anemia of chronic renal failure, the early increase in sTfR values starting recombinant human erythropoietin therapy is useful for predicting and assessing hematologic response to therapy. The change in sTfR levels occurs well before any change in hematocrit or hemoglobin values can be detected, allowing early adjustments in erythropoietin dosage and iron supplementation therapy. Moreover, a recent study indicated that the sTfR/ferritin ratio can discriminate between anemic patients with and without celiac disease. Subclinical iron deficiency in early pregnancy is strongly associated with bacterial vaginosis, and therefore sTfR and the sTfR/log ferritin index may assista identifying the risk of this condition. The sTfR/log ferritin index has also been shown to be superior to routine tests for predicting the response to iron therapy in long-term hemodialysis patients, and in discriminating between patients with iron deficiency and various hemoglobinnopathies. Serum sTfR also reflects the rate of erythroid proliferation. sTfR is therefore a useful addition to the existing anemia assays.




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