Troponin I (TnI) ELISA Test kit





TnI ELISA Test kit Intended Use
The cardiac Troponin Isoform (cTnI) ELISA test kit is intended for the quantitative determination of cardiac troponin I in human serum. Measurement of troponin I values are useful in the evaluation of acute myocardial infarction.





Troponin I ELISA Kit Components:
• Microplate: 96 wells coated with mouse monoclonal anti-TnI
• HPR- cTnI Enzyme Conjugate Reagent
• Reference Standard Set
• TMB Reagent
• Stop Solution (1N HCL)





Method Principle
This Diagnostic Automation/ Cortez Diagnostics Inc. Human serum Troponin I ELISA test is based on the principle of a solid phase enzyme-linked immunosorbent assay. The assay system utilizes four unique monoclonal antibodies directed against distinct antigenic determinants on the cTnI molecule. For further details please refer to complete instructions manual supplied with the product.





Background Information
Troponin is the inhibitory or contractile regulating protein complex of striated muscle. It is located periodically along the thin filament of the muscle and consists of three distinct proteins: troponin I, troponin C, and troponin T. The troponin I subunit exists in three separate isoforms; two in fast-twitch and slow-twitch skeletal muscle fibers, and one in cardiac muscle. The cardiac Troponin isoform (cTnI) is about 40% dissimilar. Antibodies made against this cardiac isoform are immunologically different from the other two skeletal isoforms, thus the unique isoform and tissue specificity of cardiac troponin I is the basis for its use as an aid in the diagnosis of acute myocardial infarction (AMI). The cTnI has been useful in the differential diagnosis of patients presenting to Emergency Departments (ED) with chest pain. The cTnI Enzyme Immunoassay provides a rapid, sensitive, and reliable assay for the quantitative measurement of cardiac-specific troponin I. The antibodies developed for the test will determine a minimal concentration of 1.0 ng/ml, and there is no cross-reactivity with human cardiac troponin T or skeletal troponin T or I.



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