PR-3 ELISA Test kit





PR-3 ELISA Test kit Intended Use
Proteinase-3 (PR-3) Enzyme-Linked Immunosorbent Assay (ELISA) is intended for the detection and semi-quantitative determination of antibodies to PR-3 in human sera. The results of the assay may be used as an aid in the diagnosis of Wegener’s granulomatosis.






PR3 ELISA Kit Components:
• Microplate: 96 wells coated with Proteinase-3 antigen
• HRP Conjugated Goat antihuman IgG, IgA, and IgM
• Serum Diluent Type III
• High Positive Control
• Low Positive Control
• Negative Control
• Calibrator
• Wash Buffer Type II (20X)
• Chromogen/Substrate Solution Type II (TMB)
• Stop Solution (1N H2SO4)





Method Principle
This Diagnostic Automation/ Cortez Diagnostics Inc. Human PR-3 test is an Enzyme-Linked Immunosorbent Assay to detect IgG, IgA, and IgM antibodies to PR-3 antigens. Purified PR-3 antigens are attached to microplate wells. Diluted test sera are added to each well. If the antibodies are present that recognize the antigen, antigen-antibody complexes are formed. For further details please refer to complete instructions manual supplied with the product.





Background Information
Anti-neutrophil cytoplasmic antibody (ANCA) are a group of autoantibodies. Two major fluoroscopic patterns can be recognized: a diffuse cytoplasmic staining (C-ANCA), and a perinuclear/nuclear staining (P-ANCA). In patients with vasculitis, more of 90% of C-ANCA are directed against proteinase 3 (PR3-ANCA) whereas approximately 80-90% of P-ANCA recognize myelperoxidase (MPO-ANCA). Testing for both P-ANCA and C-ANCA is highly recommended in the laboratory workup of patients who present with clinical features suggestive of SV. The clinical syndromes most frequently associated with ANCA are as follows: Wegener’s granulomatosis, Polyarteritis, “Overlap” Vasculitis, Idiopathic Crescentic Glomerulonephritis (ICGN), and Kawasaki Disease.




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