Helicobacter pylori IgG ELISA Test kit




H. pylori ELISA Test kit Intended Use
Helicobacter pylori IgG ELIZA test kit is an enzyme-linked immunosorbent assay for the qualitative detection of IgG antibodies to H. pylori in sera from symptomatic adults.




H. pylori ELISA Kit Components:
• Microplate: 96 wells coated with inactivated H. pylori antigen
• HPR-Enzyme Conjugate Goat anti-human IgG
• Sample Diluent
• Calibrator
• Positive Control
• Negative Control
• Wash Buffer (10X)
• Chromogen/Substrate Solution
• Stop Solution (1M H2SO4, 0.7MHCl)




Method Principle
This Diagnostic Automation/ Cortez Diagnostics Inc. Human H. pylori IgG ELISA test kit is designed to detect IgG class antibodies to H. pylori in human sera. Creation of the sensitized wells of the plastic microwell strips occurred using passive adsorption with H. pylori antigen. The test procedure involves three incubation steps. For further details please refer to complete instructions manual supplied with the product.





Background Information
H. pylori is usually acquired in childhood and early adulthood, although the epidemiology and transmission of H. pylori is not fully understood. Up to 50% of the world’s population is infected and many without symptoms. Developing countries display the highest infection rates. Lower socioeconomic class and crowded living conditions are associated with an increased prevalence. This Bacteria prefers to reside in the gastric mucosa and in the gastric crypts throughout the stomach, to avoid gastric acid and the mucous layer. Individuals with H. pylori present in the stomach may also have the organism in the metaplastic gastric epithelium cells of the esophagus or duodenum. Varieties of invasive and noninvasive tests are currently available for the diagnosis of H. pylori infection. The invasive diagnosis of H. pylori infection includes biopsy samples, histologic examination of stained biopsy specimens, or direct detection of the urease activity in the biopsy. Non-invasive techniques included urea breath tests and serological methods. The desirability for cost-effective and noninvasive tests to detect H. pylori infection has led to the development of methods that do not require endoscopy.



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