Gliadin IgG ELISA Test kit




Gliadin IgG ELISA Test kit Intended Use
Gliadin IgG Elisa test kit is intended for the qualitative and semi-quantitative detection of IgG-class antibodies to Gliadin in human serum. The result of this test is intended to be used as an aid in the diagnosis of gastrointestinal disorders, mainly Celiac Disease.





Gliadin IgG ELISA Kit Components:
• Microplate: 96 wells coated with inactivated antigen
• HRP Conjugated goat anti-human IgG (Fc chain specific)
• Sample Diluent
• Positive Control
• Negative Control
• Calibrator
• Wash Buffer (10X)
• TMB Substrate Solution
• Stop Solution (1M H2SO4, 0.7M HCI)





Method Principle
This Diagnostic Automation/ Cortez Diagnostics Inc. Human Gliadin IgG ELISA test system is designed to detect IgG class antibodies to Gliadin in human sera. Wells of microplate are sensitized by passive absorption with Gliadin antigen. The test procedure involves three incubation steps. For further details please refer to complete instructions manual supplied with the product.





Background Information
Gliadin is a glycoprotein within gluten and it is found in wheat and some other grains, including oats, rye, barley, and millet. Celiac disease is an inflammatory disorder of the small intestine. Immune reactions to Gliadin are likely to play a role in the pathogenesis of the disease since research demonstrates that both humoral and cell-mediated responses occur in the peripheral blood and in the gut of celiac patients.




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