Free T3 ELISA Test kit




Free T3 ELISA kit Intended Use
Free T3 ELISA test kit is designed for the quantitative determination of Free Triiodothyronine (T3) concentration in human serum.




Free T3 ELISA kit Components:
• Human Serum References
• fT3- Enzyme Reagent
• T3 Antibody Coated Microplate
• Wash Solution
• Substrate A
• Substrate B
• Stop Solution
• Stop Solution




Test Method Principle:
This Diagnostic Automation/ Cortez Diagnostics Inc. Free T3 Enzyme Immunoassay test is based on the principle of competitive immunoassay. Upon mixing immobilized antibody, enzyme-T3 conjugate and a serum containing the native free T3, a competition reaction results between the T3 free antigen and the enzyme-T3 conjugate for a limited number of insolubilized binding sites. For additional information and details please refer to the instructions for use.



Background Information
T3, a thyroid hormone, circulates in blood almost completely bound to carrier proteins with thyroxine-binding globulin (TBG) being the main carrier. However, only the free or unbound portion of T3 has biological activity. The concentrations of the carrier proteins changes in many conditions, such as pregnancy. In normal thyroid function as the concentrations of the carrier proteins alters, the total T3 level changes so that the free T3 concentration remains constant. Thus, measurements of free T3 concentrations correlate more reliably with clinical status than total T3 levels. For example, the increase in total T3 levels associated with pregnancy, oral contraceptives and estrogen therapy result in higher total T3 levels while the free T3 concentration remains unchanged. This method provides the technician with optimum sensitivity while requiring few technical manipulations in a direct determination of free T3.



Diagnostic Automation/ Cortez Diagnostics Inc. also provide other thyroid kits: T3 ELISA kit, T4 ELISA kit, TSH ELISA kit, TSH Ultra-sensitive ELISA kit, Free T3 ELISA kit, Free T4 ELISA kit and T Uptake ELISA kit. For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representatives at 818-591-3030.