Estradiol (E2) ELISA Test kit





Estradiol ELISA Test kit Intended Use
The Estradiol ELISA Kit is for the quantitative determination of Estradiol (E2) concentration in human serum.




Estradiol ELISA Kit Components:
• Microplate: 96 wells coated with goat anti-rabbit IgG
• Reference Standard Set
• Estradiol-HRP Conjugate Reagent
• Rabbit Anti-Estradiol Reagent
• Extraction and sample dilution buffer
• TMB Reagent
• Stop Solution




Method Principle
This Diagnostic Automation/ Cortez Diagnostics Inc. Estradiol ELISA Kit is a quantitative test based on the principle of competitive binding between E2 in the test specimen and E2-HRP conjugate for a constant amount of rabbit anti-Estradiol. In the incubation, goat ant-irabbit IgG-coated wells are incubated with 25 µl E2 standards, controls, patient samples. For further details please refer to complete instructions manual supplied with the product.




Background Information
Estradiol (E2) is a C18 steroid hormone, produced mainly by the ovary, placenta, and in smaller amounts by the adrenal cortex, and the male testes. Estradiol (E2) is secreted into the blood stream where 98% of it circulates bound to sex hormone binding globulin (SHBG). To a lesser extent it is bound to other serum proteins such as albumin. Only a very small fraction circulates as free hormone or in the conjugated form. Estrogenic activity is effected via estradiol-receptor complexes which trigger the appropriate response at the nuclear level in the follicles, uterus, breast, vagina, urethra, hypothalamus, pituitary and to a lesser extent the liver and skin. In non-pregnant women with normal menstrual cycles, estradiol secretion follows a cyclic, biphasic pattern with the highest concentration found immediately prior to ovulation. The rising estradiol concentration is understood to exert a positive feedback influence at the level of the pituitary where it influences the secretion of the gonadotropins, follicle stimulating hormone (FSH), and luteinizing hormone (LH), which are essential for follicular maturation and ovulation, respectively. Following ovulation, estradiol levels fall rapidly until the luteal cells become active resulting in a secondary gentle rise and plateau of estradiol in the luteal phase. During pregnancy, maternal serum Estradiol levels increase considerably, to well above the pre-ovulatory peak levels and high levels are sustained throughout pregnancy.




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