ENA Profile ELISA Test kit





ENA Profile ELISA Test kit Intended Use
The ENA Profile-6 ELISA test system is a semi-quantitative immunoassay for the detection of IgG antibodies to Jo-1, Sm, Sm/RNP, SSA(Ro), SSB(La), and Scl-70 in human sera. When performed according to these instructions, the results of this autoantibody profile may aid in the diagnosis and treatment of autoimmune connective tissue disorders





ENA Profile ELISA Kit Components:
• Microplate: 96 wells coated with inactivated antigen
Each row of the plate is coated with a different ENA
• HRP Conjugated goat anti-human IgG (Fc chain specific)
• Sample Diluent
• Positive Control
• Negative Control
• Calibrator
• Wash Buffer Type I (10X)
• TMB Substrate Solution
• Stop Solution (1M H2SO4, 0.7M HCI)





Method Principle
This Diagnostic Automation/ Cortez Diagnostics Inc. Human ENA Profile-6 ELISA test system is designed to detect IgG class antibody to different autoantigens in human sera. Wells of plastic microwell strips are sensitized by passive absorption with immobilized antigens. The test procedure involves three incubations steps. For further details please refer to complete instructions manual supplied with the product.





Background Information
In recent years it has become clear that detection of autoantibodies to a number of nuclear constituents have proven to be useful in the diagnosis of various connective tissue diseases. The exact etiology of autoimmune diseases is unknown, and the specific role played by autoantibodies in the onset of various autoimmune connective tissue diseases is not fully clear. However, the association and frequency of detection of Jo-1, Sm, Sm/RNP, SSA(Ro), SSB(La), and Scl-70 antibodies by this ENA Profile-6 ELISA test system, offers an efficient test procedure for the laboratory workup of patients with various connective tissue diseases.





For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.