Description

SSA (La) ELISA Test kit





SSA (La) ELISA Test kit Intended Use
SSB (La) test kit is a semi-quantitative immunoassay for the detection of IgG antibodies to SSB in human sera. The results of this assay may aid in the diagnosis and treatment follow up of autoimmune connective tissue disorders.





SSA (Ro) ELISA Kit Components:
• Microplate: 96 wells coated with inactivated antigen
• HRP Conjugated goat anti-human IgG
• Sample Diluent
• Positive Control
• Negative Control
• Calibrator
• Wash Buffer (10X)
• TMB Substrate Solution
• Stop Solution (1M H2SO4, 0.7M HCI)





Method Principle
This Diagnostic Automation/ Cortez Diagnostics Inc. Human SSB (La) test kit is designed to detect IgG class antibodies to SSB in human sera. Wells of plastic microplate strips are sensitized by passive absorption with SSB antigens. The test procedure involves three incubation steps. For further details please refer to complete instructions manual supplied with the product.





Background Information
Sjögren syndrome type B antigen (SS-B) also known as Lupus La protein was originally defined by its reactivity with autoantibodies from patients with Sjögren syndrome and systemic lupus erythematosus. SS-B/La is a member of an autoantigens commonly referred to as extractable nuclear antigens (ENAs) composed of protein and RNA. The other ENAs are SS-A/Ro, RNP, and Sm. SS-B/La antibodies are found primarily in 60% of patients with Sjögren syndrome and 15% of lupus erythematosus (LE). Antibodies to ENAs are common in patients with connective tissue diseases or systemic rheumatic diseases including mixed connective tissue disease, Sjögren syndrome, systemic sclerosis, LE, and polymyositis /dermatomyositis.




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