Description

Mumps ELISA Test kit




Mumps ELISA Test kit Intended Use
Mumps IgG ELISA test kit is intended for the detection and quantitative determination of IgG antibody to Mumps virus in human sera. Individual serum specimens may be used for the determination of immune status. Paired sera, acute and convalescent, may be used to demonstrate seroconversion or a significant rise in antibody, as an aid in the diagnosis of a recent or current infection.




Mumps ELISA Kit Components:
• Microplate: 96 wells coated with inactivated Mumps virus antigen
• HPR-Enzyme Conjugate Goat anti-human IgG
• Serum Diluent Type I
• Calibrator
• Positive Control
• Negative Control
• Chromogen/Substrate Solution Type I
• Wash Buffer (20X)
• Stop Solution (1N H2SO4)




Method Principle
This Diagnostic Automation/ Cortez Diagnostics Inc. Human Mumps IgG ELISA test kit is designed to detect IgG class antibodies to Mumps virus in human sera. Wells of plastic microplate strips are sensitized by passive absorption with Mumps antigen. When antigens bound to the solid phase are brought into contact with a patient serum, antigen specific antibody, if present, will bind to the antigen on the solid phase forming antigen- antibody complexes. For further details please refer to complete instructions manual supplied with the product.





Background Information
The mumps virus, the etiological agent of mumps in human, is a member of the paramyxovirus group. Mumps is one of the most common causes of aseptic meningitis, encephalitis, and inflammation of the testes (orchitis), pancreas, and ovaries. Parotitis as a presenting symptom in mumps infections is usually sufficiently diagnostic to preclude serological confirmation. However, a third of mumps infections are subclinical or unrecognized and require additional serological procedure to confirm or rule out mumps infection. ELISA can be both specific and sensitive for the detection and measurement of serum proteins. The sensitivity, specificity, and reproducibility of ELIZA can be comparable to other serological tests for antibody, such as immunofluorescence, complement fixation, hemagglutination and neutralization. ELISA is as sensitive as the neutralization test and more sensitive than CF and HAI which makes it a reliable test for determination of immune status. Our Mumps IgG ELISA kit provides all the necessary reagents for the rapid determination and quantitation of IgG antibody to mumps virus in human sera.



For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.