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Name Epstein Barr Virus Nuclear Antigen (EBNA) IgG ELISA kit
Price $410.00
Category NameInfectious Disease ELISA kits
MethodELISA: Enzyme Linked Immunosorbent Assay
PrincipleELISA - Indirect; Antigen Coated Plate
Detection RangeQualitative - Positive, Negative and Cut-off
Total Time60 min
Shelf Life12 months from the manufacturing date

Item #:                    1425-1   Quantity:               



EBV ELISA Test kit

EBV ELISA Test kit Intended Use
Epstein-Barr Virus Nuclear Antigen (EBNA-1) IgG ELISA is intended for the qualitative and semi-quantitative determination of IgG antibody in human serum to EBNA-1 recombinant antigen as an aid in the diagnosis of infectious mononucleosis.

EBV IgG ELISA Kit Components:
• Microplate: 96 wells coated with recombinant EBNA-1 antigen
• HPR-Enzyme Conjugate Goat anti-human IgG
• Sample Diluent Type I
• Calibrator
• High Positive Control
• Low Positive Control
• Negative Control
• Chromogen/Substrate Solution Type I
• Wash Buffer (20X)
• Stop Solution (1N H2SO4)

Method Principle
This Diagnostic Automation/ Cortez Diagnostics Inc. Human EBNA1 IgG ELISA test system is designed to detect IgG class antibodies to EBNA-1 recombinant antigen in human sera. When antigens bound to the microplate wells are brought into contact with a patient serum, antigen specific antibody, if present, will bind to the antigen on the solid phase forming antigen-antibody complexes. For further details please refer to complete instructions manual supplied with the product.

Background Information
Epstein-Barr Virus (EBV) is a ubiquitous human virus; classified as a member of the herpesvirus family based upon its characteristic morphology. EBV has been etiologically implicated in an increasing number of human diseases, such as infectious mononucleosis, Burkitt lymphoma, and nasopharyngeal carcinoma. By adulthood virtually everyone has been infected with and has developed immunity to the virus. In underdeveloped countries, seroconversion to the virus takes place in early childhood and is usually asymptomatic. In more affluent countries, primary EBV infections are often delayed until adolescence or later, and manifest as IM in about 50% of this age group. Following seroconversion, whether symptomatic or not, EBV establishes a chronic, latent infection in B lymphocytes which may last for life. Expression of EBNA-1 either follows or parallels membrane antigen at 12 to 24 hours post infection. Antibody levels of EBNA-1 IgG, are diagnostic in determining acute and convalescent stages in IM. IgG antibodies to EBNA-1 are rarely present in acute IM and rise during convalescence. They will rise to a plateau level in three months to a year and will normally persist for life.

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