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Name dsDNA ELISA kit
Price $410.00
Category NameAutoimmune Disease kits
MethodELISA: Enzyme Linked Immunosorbent Assay
PrincipleELISA - Indirect; Antigen Coated Plate
Detection RangeSemi-Quantitative - Positive, Negative Control
Sample10 ul serum
Total Time~ 65 min
Shelf Life12-14 Months from the manufacturing date

Item #:                    2553-1   Quantity:               



Anti-dsDNA ELISA Test kit

Anti-dsDNA ELISA Test kit Intended Use
The dsDNA Enzyme-Linked Immunosorbent Assay (ELISA) is intended for the detection of antibodies in human serum to dsDNA antigen and as an aid in diagnosis of Systemic lupus erythematosus (SLE).

Anti-dsDNA ELISA Kit Components:
• Microplate: 96 wells coated with Purified dsDNA antigen
• Serum Diluent Type II
• HRP Conjugated Goat anti-human IgG & IgM
• Positive Control
• Negative Control
• Calibrator
• Wash Buffer Type I (20X)
• TMB Chromogen/Substrate Solution
• Stop Solution (1N H2SO4)

Method Principle
This Diagnostic Automation/ Cortez Diagnostics Inc. Human dsDNA elisa test is an Enzyme-Linked Immunosorbent Assay to detect antibodies to dsDNA antigen. Purified dsDNA antigen is attached to a solid phase microassay well. When antigens are brought into contact with a patient\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\'s serum, antigen specific antibody, if present, will bind to the antigen on the solid phase forming antigen-antibody complexes. For further details please refer to complete instructions manual supplied with the product.

Background Information
Antibodies to native DNA (anti-DNA) are of considerable interest in the diagnosis and management of patients with systemic lupus erythematosus (SLE). The detection of elevated anti-dsDNA, complement-fixing ability and antigen avidity are important with respect to the pathogenesis of tissue damage and the clinical features of SLE. ELISA test for dsDNA antibodies has many advantages over the immunofluorescent method including a sensitive, specific assay in an easy to use format providing quick and efficient results. The dsDNA antigen used in this Anti-dsDNA ELISA kit is purified through modified immunoaffinity adsorption methods utilizing immobilized antigen-specific human immunoglobulins.

For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.