Description

Testosterone ELISA Test kit




Testosterone ELISA Test kit Intended Use
The Testosterone ELISA Kit or Enzyme Immunoassay (EIA) is intended for the quantitative determination of testosterone in human serum. Measurement of testosterone can be used as an aid in the diagnosis and treatment of various hormonal and sexual disorders.




Testosterone ELISA Kit Components:
• Microplate: 96 wells coated with goat anti-rabbit IgG
• Reference Standard Set
• Pregesterone -HRP Conjugate Concentrate
• Rabbit Anti-Progesterone Reagent
• Progesterone Conjugate Diluent
• Pregesterone Control 1 and 2
• TMB Reagent
• Stop Solution




Method Principle
This Diagnostic Automation/ Cortez Diagnostics Inc. Testosterone ELISA Kit is a quantitative test based on the principle of competitive binding between testosterone in the test specimen and testosterone-horseradish peroxidase (HRP) conjugate, for a constant amount of rabbit anti-testosterone. For further details please refer to complete instructions manual supplied with the product.




Background Information
Testosterone a C19 steroid, is the most potent naturally secreted androgen. Testosterone is primarily secreted by the test of males and the ovaries of females and small amounts by the adrenal glands. Testosterone plays key roles in health and wellbeing in both males and females. In the blood Testosterone is bound to SHBG (60 %) and in lower quantity to other protein such as albumin. Only the measurement of Free Testosterone (< 1% of Total Testosterone) allows the measurement of biologically active hormone. Testosterone levels decline gradually with age in men and free testosterone levels are elevated in women with hyperandrogenism associated with hirsutism in the presence or absence of polycystic ovarian disease. Moreover, free testosterone measurements may be more useful than total testosterone in situations where SHBG is increased or decreased such as in hypothyroidism and obesity.




For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.