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Name TSH ELISA kit
Price $285.00
Category NameThyroid ELISA kits
MethodELISA: Enzyme Linked Immunosorbent Assay
PrincipleSandwich Complex
Sample100 ul serum
Sensitivity0.20 uIU/mL
Total Time~ 80 min
Shelf Life12 Months from the manufacturing date

Item #:                    3122-18   Quantity:               



TSH ELISA Test kit

TSH ELISA kit Intended Use
Thyroid Stimulating Hormone (TSH) human ELISA (Enzyme-Linked Immunosorbent Assay) kit is for the quantitative measurement of human TSH concentration in serum. The assay is useful in the diagnosis of thyroid or pituitary disorders.

TSH ELISA kit Components:
• Microplate: Antibody-Coated Wells
• Enzyme Conjugate Reagent
• Reference Standard set
• TMB Reagent
• Stop Solution (1N HCl)

Test Method Principle
The Diagnostic Automation/Cortez Inc. TSH ELISA test is based on the principle of a solid phase enzyme-linked immunosorbent assay.The assay system utilizes a unique monoclonal antibody directed against a distinct antigenic epitope on the intact TSH molecule. For additional information and details please refer to the instructions for use.

Background Information
The determination of serum or plasma levels of TSH, also known as thyrotropin, is recognized as a sensitive method in the diagnosis of primary and secondary hypothyroidism. TSH is secreted by the pituitary gland and induces the production and release of thyroxine (T4) and triiodothyronine (T3) from the thyroid gland. TSH is a glycoprotein consisting of two chemically different subunits, alpha and beta. The concentration of TSH in the blood is low, but it is essential for the maintenance of normal thyroid function. The release of TSH is regulated by a TSH-releasing hormone (TRH) produced by the hypothalamus. The levels of TSH and TRH are inversely related to the level of thyroid hormone. When there is a high level of thyroid hormone in the blood, less TRH is released by the hypothalamus, so less TSH is secreted by the pituitary. The opposite action will occur when there is decreased thyroid hormone in the blood (negative feedback). TSH and the pituitary glycoproteins: luteinizing hormone (LH), follicle stimulating hormone (FSH), and human chorionic gonadotropin (HCG), have identical alpha chains. These regions of homology can cause considerable cross-reactivity with some polyclonal TSH antisera. Each of these hormones also has a unique beta subunit, which provides receptor specificity. The use of a monoclonal antibody in Diagnostic Automation, Inc. TSH ELISA test eliminates this interference, which could result in falsely elevated TSH values in either menopausal or pregnant females, a population whose evaluation of thyroid status is clinically significant.

Diagnostic Automation/ Cortez Diagnostics Inc. also provide other thyroid kits: T3 ELISA kit, T4 ELISA kit, TSH ELISA kit, TSH Ultra-sensitive ELISA kit, Free T3 ELISA kit, Free T4 ELISA kit and T Uptake ELISA kit. For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representatives at 818-591-3030.