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Name T Uptake (T3-Uptake) ELISA kit
Price $184.00
Category NameThyroid ELISA kits
MethodELISA: Enzyme Linked Immunosorbent Assay
PrincipleCompetative ELISA
Detection Range18 - 47% Uptake
Sample50 uL
Total Time~75 min
Shelf Life12 months

Item #:                    3176-15   Quantity:               



T3- Uptake ELISA kit

T3- Uptake ELISA kit Intended Use
Thyroid hormone uptake (T-Uptake or T3-Uptake) test is used for an indirect measurement of the total thyroid binding proteins capacity in blood.

T3-Uptake ELISA kit Components:
• Human Serum References
• T3 Uptake Enzyme Reagent
• T3-Uptake Conjugate Buffer
• T4 Antibody Coated Plate
• Wash Solution
• Substrate A
• Substrate B
• Stop Solution
• Product Instructions

Test Method Principle
This Diagnostic Automation/ Cortez Diagnostics Inc. Triiodothyronine (T3)-Uptake test indirectly measures the binding capacity of thyroid binding proteins (Thyroid Binding Pre-Albumin or TBPA, Albumin, and Thyroxine Binding Globulin or TBG) in plasma. T3 Uptake in the sample is directly proportional to the degree of saturation of the binding proteins by thyroxine (T4). In this method enzyme-T3 conjugate and T4 are added, and then the reactants are mixed. The endogenous binding proteins of the sample react with the T4, but not with the enzyme T3-conjugate. This leads to a higher binding of the enzyme-T3 conjugate to the antibody sites, reactive for both T3 and T4, immobilized on the well as the binding capacity of the specimen increases. After the incubation period, the antibody bound enzyme-T3 conjugate is separated from the unbound portion. The activity of the enzyme present on the surface of the well is quantitated by reaction with a suitable substrate to produce color is directly correlated with thyroid hormone binding capacity.

Background Information
The thyroid gland produces and secretes T4 and T3 into the general circulation. The released hormones do not circulate as free molecules but are almost entirely bound to specific serum proteins. Three protein fractions with varying affinities and capacities for interaction with T3 and T4 have been identified as TBG, TBPA and Albumin. Since the metabolic processes are regulated entirely by the concentration of the free thyroid hormones, which are inversely related to the levels of the binding proteins, an assessment of the binding capacity of human serum was developed.

Diagnostic Automation/ Cortez Diagnostics Inc. also provide other thyroid kits: T3 ELISA kit, T4 ELISA kit, TSH ELISA kit, TSH Ultra-sensitive ELISA kit, Free T3 ELISA kit, Free T4 LIS kit and T Uptake Elisa kit. . For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representatives at 818-591-3030.