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Name MPO ,Myeloperoxidase (p-ANCA) ELISA Kit
Price $475.00
Category NameAutoimmune Disease kits
MethodELISA: Enzyme Linked Immunosorbent Assay
PrincipleSandwich Complex
Detection RangeQualitative & Semi - Quantitative Positive, negative, cut off
Sample10 ul serum
Total Time~60 min
Shelf Life12 Months from the manufacturing date

Item #:                    1441-2   Quantity:               



MPO ELISA Test kit

MPO ELISA Test kit Intended Use
Myeloperoxidase (MPO) Elisa test kit is intended for the qualitative and semi-quantitative detection of IgG-class antibody to myeloperoxidase in human serum. The result from MPO elisa test can be used as an aid in the diagnosis of various autoimmune vasculitic disorders characterized by elevated levels of anti-neutrophil cytoplasmic antibodies (ANCA). MPO-ANCA may be associated with autoimmune disorders such as Wegener’s granulomatosis, ICGN, MPA and PRS.

MPO ELISA Kit Components:
• Microplate: 96 wells coated with inactivated Myeloperoxidase antigen
• HRP Conjugated goat anti-human IgG (Fc chain specific)
• Sample Diluent
• Positive Control
• Negative Control
• Calibrator
• Wash Buffer (10X)
• TMB Substrate Solution
• Stop Solution (1M H2SO4, 0.7M HCI)

Method Principle
This Diagnostic Automation/ Cortez Diagnostics Inc. Human Myeloperoxidase (MPO) ELISA test kit is designed to detect IgG class antibodies to MPO in human sera. Wells of microplate are sensitized by passive absorption with MPO antigen. The test procedure involves three incubation steps. For further details please refer to complete instructions manual supplied with the product.

Background Information
Since the initial discovery of Anti-neutrophil cytoplasmic antibody (ANCA), scientists have associated ANCA with a number of Systemic Vasculitides (SV). ANCA includes two primary specificities: C-ANCA directed against Proteinase-3 (PR-3), and P-ANCA directed against Myeloperoxidase (MPO). Testing for both P-ANCA and C-ANCA is highly recommended in the laboratory workup of patients who present with clinical features suggestive of SV. The clinical syndromes most frequently associated with ANCA are as follows: Wegener’s granulomatosis, Polyarteritis, “Overlap” Vasculitis, Idiopathic Crescentic Glomerulonephritis (ICGN), and Kawasaki Disease.

For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.