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Name Lyme Disease IgM ELISA kit
Price $515.00
Category NameInfectious Disease ELISA kits
MethodELISA: Enzyme Linked Immunosorbent Assay
PrincipleELISA - Peroxidase conjugated
Detection RangeQualitative - Positive, Negative and Cut-off
Sample10ul serum
Total Time~ 70 min
Shelf Life12 Months from the manufacturing date

Item #:                    1424-2   Quantity:               



B. burgdorferi IgM (Lyme) ELISA Test kit

Lyme disease ELISA Test kit Intended Use
Borrelia burgdorferi IgM ELISA kit is an ELISA for the qualitative detection of IgM antibodies to Borrelia burgdorferi in human serum. This ELISA test kit should be used for patients with signs and symptoms that are consistent with Lyme disease and can be used to support a clinical diagnosis of Lyme disease

Lyme disease ELISA Kit Components:
• Microplate: 96 wells coated with inactivated B. burgdorteri B31 antigen
• HPR-Enzyme Conjugate Goat anti-human IgM
• Serum Diluent
• Calibrator
• Positive Control
• Negative Control
• Stop Solution (1M H2SO4, 0.7M HCI)
• Wash Buffer (10X)

Method Principle
This Diagnostic Automation/ Cortez Diagnostics Inc. Human Borrelia burgdorferi IgM ELISA test system is designed to detect IgM class antibodies to B. burgdorferi IgM in human sera for the detection of Lyme disease. Wells of plastic micro well strips are sensitized by passive absorption with B. burgdorferi IgM antigen. The test procedure involves three incubation steps. For further details please refer to complete instructions manual supplied with the product.

Background Information
Lyme disease occurs in three stages, often with intervening latent periods and with different clinical manifestations. Ticks of the genus Ixodes transmit the Borrelia burgdorferi which is a spirochete that causes Lyme disease. In endemic areas, these ticks reside on vegetation and animals such as deer, mice, dogs, horses, and birds. Skin is the portal of entry for B. burgdorferi and the tick bite often causes a characteristic rash called erythema migrans (EM). Symptoms vary according to the sites affected by the infection such as joints, skin, central nervous system, heart, eye, bone, spleen, and kidney. Late disease is most often associated with arthritis or CNS syndromes. Asymptomatic subclinical infection is possible and infection may not become clinically evident until the later stages. Patients with early infection produce IgM antibodies during the first few weeks after onset of EM and produce IgG antibodies more slowly. Tests only detect IgM during the first month after onset of illness. The majority of patients develop IgG antibodies within one month. Both IgG and IgM antibodies can remain detectable for years.

For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.