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Name Legionella G/M/A ELISA kit
Price $410.00
Category NameInfectious Disease ELISA kits
Test96
MethodELISA: Enzyme Linked Immunosorbent Assay
PrincipleELISA - Peroxidase conjugated
Detection RangeQualitative - Positive, Negative and Cut-off
Sample10ul
Specificity95%
Sensitivity95%
Total Time~ 70 min
Shelf Life12 Months from the manufacturing date

Item #:                    1651-2   Quantity:               

 
   




 Description


Legionella ELISA Test kit




Legionella ELISA Test kit Intended Use
Legionella pneumophila IgG/IgM/IgA ELISA is intended for the qualitative detection of total antibodies (IgG/IgM/IgA) to Legionella pneumophila (Legionella) serogroups 1-6 in human serum from patients with clinical suspicion of Legionella Disease.




Legionella ELISA Kit Components:
• Microplate: 96 wells coated with inactivated Legionella antigen
• HPR-Enzyme Conjugate Goat anti-human IgG/ IgM/IgA
• Sample Diluent
• Calibrator
• Positive Control
• Negative Control
• Wash Buffer (10X)
• Chromogen/Substrate Solution
• Stop Solution (1M H2SO4, 0.7MHCl)




Method Principle
This Diagnostic Automation/ Cortez Diagnostics Inc. Human Legionella IgG/IgM/IgA ELISA test system is designed to detect antibodies to L. pneumophila in human sera. Wells of plastic microwell strips are sensitized by passive absorption with Legionella antigen. The test procedure involves three incubation steps. For further details please refer to complete instructions manual supplied with the product.




Background Information
Legionella pneumophila is the causative agent for Legionellosis (Legionella pneumonia, or Legionnaire Disease). Presently, there are more than 25 species and 33 serogroups in the family Legionellaceae, with at least 18 species associated with pneumonia, accounting for roughly 1-5% of all cases of pneumonia. The symptoms of Legionellosis range from subclinical asymptomatic infections to severe life-threatening pneumonia. Optimum times for specimen collection appear to be within the first week of illness, or as soon as possible after the onset (acute specimen), and at least 3 weeks after the onset (convalescent specimen).This ELISA test kit utilizes a mixture of Legionella pneumophila serogroups 1-6 as the antigen. The antibody response to Legionella pneumophila can be quite diverse. Individuals not only attain varied titers, but also give heterological response to different serotypes and species. The heterologic response is variable and is probably characteristic of the individual and does not seem to be mediated by the antigenic nature of the infecting strain.


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