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Name Histamine ELISA kit
Price $820.00
Category Name

Item #:                    5133-8   Quantity:               



Histamine ELISA Test kit

Histamine ELISA Test kit Intended Use
Histamine Food ELISA Kit that is an Enzyme Immunoassay for the quantitative determination of histamine in Food. This Histamine Food ELISA Kit is a quick, economical, and highly sensitive Food Toxin ELISA Kit detection system to detect histamine in food.

Histamine ELISA Kit Components:
• Microplate: 96 wells coated with histamine conjugate
• Sample Diluent
• Histamine Standards
• Reaction Solution
• Neutralizing Solution
• Anti-Histamine Antibody
• Conjugate: anti-mouse-IgG-HRP
• Substrate Solution, TMB
• Stop Solution
• Washing Solution

Method Principle
This Diagnostic Automation/ Cortez Diagnostics Inc. Food Histamine Food ELISA Kit is a quantitative test based on the principle of the enzyme linked immunosorbent assay. A histamine conjugate is bound on the surface of a microplate wells. Samples or standards and an antibody directed against histamine are given into the wells. Immobilized and free histamine compete for the antibody binding sites. For further details please refer to complete instructions manual supplied with the product.

Background Information
Histamine is a biogenic amine, which is formed from the amino acid histidine. Histamine is produced in the mast cells and basophilic white cells. In the course of a type I allergy, endogenic histamine from the mast cells and basophilic leucocytes is released after antigen binding to membrane-associated IgE, and typical allergical reactions appear. Histamine can also enter the human body via the nutrition and can thus cause pseudoallergic food intolerances. The appearing symptoms are not distinguishable from those of a real allergy. The term, food allergy is however only used, if the pathologic symptoms develop in the course of an immunological reaction. Non-immunological reactions to food are defined as food intolerances. Food intolerances, which are caused by increased histamine concentrations, are clinically characterized by rash, diarrhea, vomiting, nausea, itching, headache and asthma. The extent of the reaction is dependent on the ingested amount of histamine. These reactions can be enhanced by a lack of diaminoxidase. Separated from histamine intolerances toxic reactions exist, which are caused by very high histamine concentrations. Toxic histamine concentrations, which can cause the so-called scombroid reaction, appear after bacterial degradation of protein-rich food, especially fish. Histamine is found above all in cheese, fish, spinach, smoked products, and red wine. After an appropriate sample preparation on this Histamine Food ELISA Test Kit, 40 samples can be tested in duplicate within 80 minutes.

For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.