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| Name | ENA Combined ELISA kit |
|---|---|
| Price | $780.00 |
| Category Name | Autoimmune Disease kits |
| Test | 96 |
| Method | ELISA: Enzyme Linked Immunosorbent Assay |
| Principle | ELISA - Peroxidase conjugated |
| Detection Range | Semi-Quantitative- Positive, Negative and Cut-off |
| Sample | 10ul serum |
| Specificity | 100% |
| Sensitivity | 93% |
| Total Time | ~ 65 min |
| Shelf Life | 12 Months from the manufacturing date |
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Description
ENA Combined Screen ELISA Test kit
ENA Combined Screen ELISA Test kit Intended Use
The ENA Screen ELISA Test kit is a qualitative screening assay designed to detect antibodies to extractable nuclear antigens (anti-ENA) in human sera. When performed according to the enclosed instructions, this test system is capable of detecting all anti-ENAs commonly tested for, such as those against Jo-1,Sm,Sm/RNP, SSA, SSB, and Scl- 70.
Kit Components:
• Microplate: 96 wells coated with inactivated antigen
• HRP Conjugated goat anti-human IgG (Fc chain specific)
• Sample Diluent
• Positive Control
• Negative Control
• Calibrator
• TMB Substrate Solution
• Stop Solution (1M H2SO4, 0.7M HCI)
• Wash Buffer (10X)
Method Principle
This Diagnostic Automation/ Cortez Diagnostics Inc. Human ENA Screen ELISA test system is designed to detect IgG class antibodies to a variety of common nuclear antigens in human sera. Wells of plastic microwell strips are sensitized by passive absorption with ENA antigen. The test procedure involves three incubation steps. For further details please refer to complete instructions manual supplied with the product.
Background Information
In recent years it has become clear that detection of autoantibodies to a number of nuclear constituents have proven to be useful in the diagnosis of various connective tissue diseases. The exact etiology of autoimmune diseases is unknown, and the specific role played by autoantibodies in the onset of various autoimmune connective tissue diseases is not fully clear. However, the association and frequency of detection of Jo-1, Sm, Sm/RNP, SSA(Ro), SSB(La), and Scl-70 antibodies by this ENA Profile-6 ELISA test system, offers an efficient test procedure for the laboratory workup of patients with various connective tissue diseases.
For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.
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