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Name Cardiolipin IgM ELISA kit
Price $550.00
Category NameAutoimmune Disease kits
MethodELISA: Enzyme Linked Immunosorbent Assay
PrincipleIndirect : Antigen Coated Plate
Detection RangeSemi-Quantitative elisa assay- Positive, Negative and Cut-off
Sample10 ul serum
Total Time~60min
Shelf Life12 Months from the manufacturing date

Item #:                    1492-2   Quantity:               



Cardiolipin IgM ELISA Test kit

Cardiolipin IgM ELISA Test kit Intended Use
Cardiolipin Antibody Elisa test kit is an enzyme linked immunosorbent assay (ELISA) designed for the semi-quantitative measurement of circulating IgM autoantibodies to cardiolipin.

Kit Components:
• Microplate: 96 wells coated with Cardiolipin antigen
• Peroxidase Conjugated goat anti-human IgM
• Positive Control (Human Serum)
• Calibrator (Human Serum)
• Negative Control (Human Serum)
• Cardiolipin IgM Test Kit Sample Diluent
• TMB Substrate Solution
• Stop Solution (1M H2SO4, 0.7M HCL)
• Wash Buffer Concentrate (10X)

Method Principle
This Diagnostic Automation/ Cortez Diagnostics Inc. Human Cardiolipin IgM ELISA Test kit is designed to detect IgM class antibodies to Cardiolipin in human sera. Wells of microplate strips are sensitized by passive absorption with Cardiolipin antigen. The test procedure involves three incubation steps. For further details please refer to complete instructions manual supplied with the product.

Background Information
Cardiolipin auto antibodies (ACA) or anti-cardiolipin (aCL) are frequently found in patients with systemic lupus erythematosus (SLE) as in patients with other autoimmune diseases, and in some individuals with no apparent chronic illnesses. Elevated levels of ACA/aCL have been reported to be significantly associated with the presence of both venous and arterial thrombosis, thrombocytopenia, and recurrent fetal loss. Anti-phospholipid syndrome has been used to describe patients who present these clinical manifestations, in association with ACA or lupus anticoagulant. ACAs are found in the immunoglobulin classes IgG, IgM and IgA. The determination of IgM antibodies is a valuable indicator in the diagnosis of beginning of the autoimmune disease, whereas IgG antibodies will be found in progressive stages of manifested autoimmune disorders. ELISA testing for ACAs of various isotypes aids in diagnosis of anti-phospholipid syndrome in patients with SLE and lupus-like disorders.

For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.