The test kit contains microtiter strips each with 8 break-off reagent wells coated with cardiolipin. In the first reaction step, diluted patient samples are incubated in the wells. In many cases, antibodies to cardiolipin rely on a plasma protein (β2-glycoprotein I) as a cofactor for antigen recognition. The coating of the microplate and the sample buffer of this ELISA therefore contain this cofactor. In the case of positive samples, the specific IgAGM antibodies will bind to the antigens. To detect the bound antibodies, a second incubation is carried out using an enzyme-labelled anti-human IgAGM (enzyme conjugate) catalyzing a color reaction.
For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.