Description

Diagnostic Automation/Cortez Diagnostics, Inc. offers an E. coli Verotoxin Food ELISA Kit that is an Enzyme-linked immunosorbent assay that may be used to screen food products for the presence of Verotoxin. This Verotoxin Food ELISA Kit is a quick, economical, and sensitive Food Toxin ELISA Kit which is designed to detect verotoxin antigen in food.  The Verotoxin Food ELISA Kit represents a highly sensitive Food ELISA Test Kit detection system and is particularly capable of the quantification of verotoxin in food products.

 

The Verotoxin Food ELISA Kit is a quantitative test based on the principle of the enzyme-linked immunosorbent assay.  During the first incubation, Verotoxin antigen in the sample is captured by antibodies attached to the wells of the Verotoxin ELISA Kit.  The second incubation adds an additional anti-Verotoxin antibody cocktail that “sandwiches” the antigen in the Verotoxin ELISA Kit.  The next incubation attaches horseradish peroxidase to the sandwich in the Verotoxin ELISA Kit.  After washings to remove unbound enzyme on the Verotoxin ELISA test, a chromogen is added which develops a blue color in the presence of the enzyme complex and peroxide. The stop solution in the Verotoxin ELISA test ends the reaction and turns the blue color to yellow.

The Verotoxin (VT), or shiga-like toxin family is a group of closely related toxins produced by certain strains of Escherichia coli. These strains are a significant cause of human hemorrhagic colitis (HC) and hemolytic uremic syndrome (HUS). In addition, they are both water borne and food borne and may also be transmitted from person-to-person by the oral-fecal route. In adults, illness caused by Verotoxin may last several days, while in children and the elderly, the illness can be fatal. Infections occur as sporadic illnesses or outbreaks, and are distributed throughout North and South America, Europe, and Asia. The peak incidence of infection occurs during summer months. In the past, lengthy and cumbersome diagnostic procedures have been utilized for the detection of verotoxin. Currently, simple to perform enzyme-linked immunosorbent assays (ELISA), like this Verotoxin Food ELISA Test Kit, are being used as an alternative to these longer tedious methods. These antigen-detection assays provide a cost effective tool for the management of diarrheal disease with an accurate and rapid test.

 

Diagnostic Automation/Cortez Diagnostics, Inc. offers several popular Food ELISA Test Kits:   Chloramphenicol ELISA Kit, Fumonisin ELISA Kit, Aflatoxin B1 ELISA Kit, Aflatoxin M1 ELISA Kit, Histamine ELISA Kit, Streptomycin ELISA Kit, and Penicillin ELISA Kit.  For more information on any of these Food ELISA Test Kits, please contact our Customer Service Department.