Description

The Diagnostic Automation, Inc. Trypanosoma cruzi- Schistosoma ELISA Kit is used for the qualitative determination of serum antibodies in humans primarly in IgG.

During the first incubation, the antibodies in the patients’ serum bind to the antigens in the test well. The next incubation allows the enzyme complex to bind to the antigen-antibody complex. After a few washings to remove unbound enzymes, a substrate is added that develops a blue color in the presence of the enzyme complex and peroxide. The stop solution ends the reaction, turning the blue assay color to yellow.

A sample should be interpreted as positive if the degree of color development is obvious and significant. Read all wells using a bichromatic reading with filters at 450 nm and 620-650 strong>Positive - Absorbance reading greater or equal to 0.2 OD units.
strong>Negative - Absorbance reading less than 0.2 OD units.
The use of a positive and negative control allows easy validation of kit stability. For a valid test, the positive control must be over 0.5 OD units and the negative control must be under 0.2 units. Should the values fall outside these ranges, the kit should not be used.
Serological results should be used as an aid in diagnosis and should not be interpreted as diagnostic by themselves.