Description

Diagnostic Automation/Cortez Diagnostics, Inc. offers a Peanut ELISA Kit that is an Enzyme Immunosorbent Assay for the Qualitative Determination of Peanut in food. This Peanut ELISA Kit is a rapid and sensitive Food Allergen ELISA Kit which is designed to provide a sensitive and convenient method for screening food samples for peanut residues.  The Peanut ELISA Kit represents a highly sensitive detection system and is particularly capable of the quantification of peanut residues in cookies, cereals, ice cream, and chocolate.

 

The Peanut ELISA Kit is a quantitative test is based on the principle of the enzyme linked immunosorbent assay.  An antibody directed against peanut proteins is bound on the surface of a microtiter plate on the Peanut ELISA Kit.  Peanut containing samples or standards are given into the wells of the microtiter plate on the Peanut ELISA Kit.  After 20 minutes incubation at room temperature, the wells of the Peanut ELISA Kit are washed with diluted washing solution to remove unbound material. A peroxidase conjugated second antibody directed against peanut proteins is given into the wells of the Peanut ELISA test and after 20 minutes of incubation the plate is washed again. A substrate solution is added and incubated for 20 minutes, resulting in the development of a blue color on the Peanut ELISA Kit. The color development is inhibited by the addition of a stop solution on the Peanut ELISA Kit, and the color turns yellow. The yellow color is measured photometrically at 450 nm. The concentration of peanut on the Peanut ELISA test is directly proportional to the color intensity of the Peanut ELISA test sample.

Peanut (Arachis hypogaea) belongs to the legumes. With 25 %, the fraction of proteins in peanuts is very high. Many of these proteins are known for being allergenic, such as Arachins and Conarachins which are contained in relative high amounts. For this reason peanut represents one of the most important food allergens. For peanut allergic persons, hidden peanut allergens in food are a critical problem.  Already very low amounts of peanuts can cause allergic reactions, which may lead to anaphylactic shock in severe cases. Because of this, peanut allergic persons must strictly avoid the consumption of peanuts or peanut containing food. Cross-contamination, mostly in consequence of the production process is often noticed. The chocolate production process is a representative example. This explains why in many cases the existence of peanut residues in foods cannot be excluded.  For this reason sensitive detection systems, like this Peanut ELISA Kit,  for peanut residues in foodstuffs are required.

Diagnostic Automation/Cortez Diagnostics, Inc. offers several popular Food Allergen ELISA Kits:  Egg Protein Residue in Food ELISA Kit, Soy Protein Residue Detection in Food ELISA Kit, Hazelnut ELISA Kit, Lupine ELISA Kit, Mustard ELISA Kit, Soy ELISA Kit, Almond ELISA Kit, Gluten ELISA Kit, Walnut ELISA Kit, and Milk Protein Residue Detection in Food ELISA Kit.  For more information on any of these Food Allergen ELISA Kits, please contact our Customer Service Department.