Description

Diagnostic Automation/Cortez Diagnostics, Inc. offers a Lupine ELISA Kit that is an Enzyme Immunoassay for the Quantitative Determination of Lupine/Lupin in Food.  This Lupine ELISA Kit is a rapid and sensitive Food Allergen ELISA Kit which is designed to provide a sensitive and convenient method for screening food samples for lupine/lupin residues. This Lupine ELISA Kit represents a highly sensitive Food Allergen ELISA Kit detection system for lupine and is particularly capable of the quantification of residues in sausage, bakery products, potato products, ketchup, and juices.  Due to high cross-reactivity, the Lupine ELISA kit is suitable for the detection of all commonly used lupine species.

 

The Lupine ELISA test is a quantitative test based on the principle of the enzyme linked immunosorbent assay.  An antibody directed against lupine proteins is bound on the surface of a microtiter plate on the Lupine ELISA Kit.  Lupine containing samples or standards are given into the wells of the microtiter plate on the Lupine ELISA Kit.  After 20 minutes incubation at room temperature, the wells of the Lupine ELISA test are washed with diluted washing solution to remove unbound material.  A peroxidase conjugated second antibody directed against lupine proteins is given into the wells of the Lupine ELISA Kit, and after 20 minutes of incubation, the plate on the Lupine ELISA Kit is washed again.  A substrate solution is added and incubated for 20 minutes, resulting in the development of a blue color on the Lupine ELISA test.  The color development is inhibited by the addition of a stop solution on the Lupine ELISA Kit, and the color turns yellow.  The yellow color is measured photometrically at 450 nm.  The concentration of lupin on the Lupine ELISA test is directly proportional to the color intensity of the Lupine ELISA test sample.

 

Lupine/Lupin belongs to the legume family. With about 35-45%, the fraction of proteins in lupine seed is very high. Some of these proteins, predominantly those in the α-, β- and γ-Conglutin fractions are known for being allergenic, due to cross-reaction to allergens of soy and peanut.  Because of its convenient nutritional properties the use of lupine flour as a food ingredient is highly increasing.  Lupine is often used in dietary products, especially for milk allergic people, vegetarians and patients with celiac disease. In food production three (sweet) lupin species are commonly used: Lupinus albus, Lupinus angustifolius and Lupinus luteus.

For lupine allergic persons, hidden lupine allergens in food are a critical problem. Already very low amounts of lupine can cause allergic reactions, which may lead to anaphylactic shock in severe cases. Because of this, lupine allergic persons must strictly avoid the consumption of lupine containing food. Cross- contamination, mostly in consequence of the production process, is often noticed. This explains why in many cases the existence of lupine residues in food cannot be excluded. For this reason sensitive detection systems, like this Lupine ELISA Kit, for lupine residues in foodstuffs are required.

Diagnostic Automation/Cortez Diagnostics, Inc. offers several popular Food Allergen ELISA Kits:  Egg Protein Residue in Food ELISA Kit, Soy Protein Residue Detection in Food ELISA Kit, Hazelnut ELISA Kit, Mustard ELISA Kit, Peanut ELISA Kit, Soy ELISA Kit, Almond ELISA Kit, Gluten ELISA Kit, Walnut ELISA Kit, and Milk Protein Residue Detection in Food ELISA Kit.  For more information on any of these Food Allergen ELISA Kits, please contact our Customer Service Department.