Description

Diagnostic Automation/Cortez Diagnostics, Inc. offers a Histamine Food ELISA Kit that is an Enzyme Immunoassay for the Quantitative Determination of Histamine in Food.  This Histamine Food ELISA Kit is a quick, economical, and sensitive Food Toxin ELISA Kit which is designed to detect histamine in food.  The Histamine Food ELISA Kit represents a highly sensitive Food ELISA Test Kit detection system and is particularly capable of the quantification of Histamine in food products.

 

The Histamine Food ELISA Kit is a quantitative test based on the principle of the enzyme linked immunosorbent assay. A histamine conjugate is bound on the surface of a microtiter plate of the Histamine ELISA Kit.  Samples or standards containing derivatized histamine and an antibody directed against histamine are given into the wells of the microtiter plate on the Histamine ELISA Kit.  Immobilized and free histamine compete for the antibody binding sites. After 30 minutes incubation at room temperature, the wells of the Histamine ELISA Kit are washed with diluted washing solution to remove unbound material. A peroxidase conjugate directed against the histamine antibody is given into the wells of the Histamine ELISA test and after another 30 minutes incubation, the plate is washed again. Then a substrate solution is added to the Histamine ELISA test and incubated for 20 minutes, resulting in the development of a blue color. The color development on the Histamine ELISA test is inhibited by the addition of a stop solution, and the color turns yellow. The yellow color is measured photometrically at 450 nm. The concentration of histamine on the Histamine ELISA Kit is indirectly proportional to the color intensity of the Histamine ELISA test sample.

 

Histamine is a biogenic amine, which is formed by enzymatical decarboxylation from the amino acid histidine. It occurs in the mast cells and basophilic white cells bound to heparin. In the course of a type I allergy, endogenic histamine from the mast cells and basophilic leucocytes is released after antigen binding to membrane-associated IgE, and typical allergical reactions appear. But histamine can also enter the human body via the nutrition and can thus cause pseudoallergic food intolerances. The appearing symptoms are not distinguishable from those of a real allergy. The term ‘food allergy’ is however only used, if the pathologic symptoms develop in the course of an immunological reaction. Non-immunological reactions to food are defined as food intolerances. Food intolerances, which are caused by increased histamine concentrations, are clinically characterized by rash, diarrhea, vomiting, nausea, itching, headache and asthma. The extent of the reaction is dependent on the ingested amount of histamine. These reactions can be enhanced by a lack of diaminoxidase.

 

Separated from histamine intolerances toxic reactions exist, which are caused by very high histamine concentrations. Toxic histamine concentrations, which can cause the so-called scombroid reaction, appear after bacterial degradation of protein-rich food, especially fish. Histamine is found above all in cheese, fish, spinach, smoked products and red wine. The histamine concentrations in different types of food, but also in the same food show frequently great differences. In different types of cheese there can e.g. be found histamine contents between 600 and 1000 mg/kg.  After an appropriate sample preparation on this Histamine Food ELISA Test Kit, 40 samples can be tested in duplicate within 80 minutes.

 

Diagnostic Automation/Cortez Diagnostics, Inc. offers several popular Food ELISA Test Kits:   Chloramphenicol ELISA Kit, Fumonisin ELISA Kit, Aflatoxin B1 ELISA Kit, Aflatoxin M1 ELISA Kit, Streptomycin ELISA Kit, Penicillin ELISA Kit, and Verotoxin Antigen in Food ELISA Test Kit.  For more information on any of these Food ELISA Test Kits, please contact our Customer Service Department.