Description

Diagnostic Automation/Cortez Diagnostics, Inc. offers a Fumonisin Food ELISA Kit, that is an Enzyme Immunoassay for the Quantitative Determination of Fumonisin in food.  This Fumonisin Food ELISA Kit is a rapid and sensitive Food Toxin ELISA Kit which is designed to provide a sensitive and convenient method for screening food samples for Fumonisin residue.  The Fumonisin Food ELISA Test Kit represents a highly sensitive Food ELISA Test Kit detection system and is particularly capable of the quantification of Fumonisin in Maize and Grain Products.

 

The Fumonisin Food ELISA Kit is a quantitative test based on the principle of the enzyme linked immunosorbent assay. A microtiter plate on the Fumonisin ELISA kit is coated with antibodies raised against mouse immunglobulins. The standards and samples respectively are pipetted together with a fumonisin- peroxidase conjugate and a mouse-anti-fumonisin antibody into the appropriate wells of the Fumonisin ELISA Kit.  The conjugate competes with the fumonisin of samples/standards for the limited number of antibody sites. Simultaneously the anti-fumonisin antibody is bound to the anti-mouse antibody coated on the microtiter plate of the Fumonism ELISA Kit.  After one hour incubation at room temperature, the wells of the Fumonisin ELISA Kit are washed with diluted washing solution to remove unbound material.  A substrate solution is added and incubated for 20 minutes, resulting in the development of a blue color on the Fumonisin ELISA test. The color development on the Fumonism ELISA test is inhibited by the addition of a stop solution, and the color turns yellow. The yellow color is measured photometrically at 450 nm. The concentration of fumonisin on this Fumonisin ELISA test kit is indirectly proportional to the color intensity of the Fumonisin ELISA test sample.

Fumonisin B1 and B2 are mycotoxins, which are produced by Fusarium moniliforme, F. solani and other types of fusarium. These toxins show an extreme stability against high temperatures (up to 100°C), and they can remain active in contaminated food for years. Fumonisin can be found in maize, oats and other types of grain. Worldwide a contamination in maize of 60% has been detected. When ingested by animals, fumonisin leads to neurotoxicity, hepatotoxicity and lung edema, mainly in horses and pigs. Therapeutic measures are the change of the grain given to the animals, or the administration of diuretic drugs.  In human patients, hints for the appearance of esophagus cancer could be associated with the exposition to fumonisin B1. Values assessed for the acute toxicity are 8 mg per kg weight and for the chronic situation 25 mg/kg in feed stuff. These are relatively high concentrations, but according to the Good Manufacturing Practice, the content of fumonisin B1 in food intended for humans should not exceed 1 mg/kg. The usual methods for detection of fumonisin in maize are liquid and thin-layer chromatography. The Fumonisin Food ELISA test kit is very sensitive and allows the detection of trace amounts below 25 μg per kg.

Diagnostic Automation/Cortez Diagnostics, Inc. offers several popular Food ELISA Test Kits:  Chloramphenicol ELISA Kit, Streptomycin ELISA Kit, Aflatoxin M1 ELISA Kit, Aflatoxin B1 ELISA Kit, Penicillin ELISA Kit, Histamine ELISA Kit, and Verotoxin Antigen in Food ELISA Test Kit.  For more information on any of these Food ELISA Test Kits, please contact our Customer Service Department.