Description

The Diagnostic Automation, Inc. Direct Immunoenzymatic DHEA-S Dehydropiandrosterone Sulphate ELISA kit is competitive immunoenzymatic colorimetric method for quantitative determination of DHEA-S concentration in serum and plasma.

Dehydropiandrosterone Sulphate (DHEA-S) is a natural steroid hormone found atop of the kidneys in the human body. DHEA-S derived from enzymatic conversion of DHEA in adrenal and extradrenal tissues. DHEA-S is also produced in the gonads, adipose tissue and the brain. It is the most abundant hormone in the human body and it is precursor of all sex steroids. As most DHEA-S is produced by the zona reticularis of the adrenal, it is argued that there is a role in the immune and stress response. DHEA-S may have more biologic roles. Its production in the brain suggests that is also has a role as a neurosteroid. Measurement of serum DHEA-S is a useful marker of adrenal androgen synthesis. Abnormally low levels may occur in have been reported in hypoadrenalism, while elevated levels occur in several conditions, e.g. virilizing adrenal adenoma and carcinoma, 21-hydroxylase and 3ß-hydroxysteroid dehydrogenase deficiencies and in some cases of female hirsutism. Women with polycystic ovary syndrome tend to have normal or mildly elevated levels of DHEAS. As very little DHEA-S is produced by the gonads, measurement of DHEA-S levels may aid in the localization of androgen source in virilizing conditions. DHEA-S levels show no diurnal variation.

Dehydropiandrosterone Sulphate(antigen) in the sample competes with horseradish peroxidase dehydropiandrosterone sulphate (enzyme-labeled antigen) for binding onto the limited number of antidehydroepiandrosterone sulphate (antibody) sites on the microplates (solid phase). After incubation, the bound/free separation is performed by a simple solid-phase washing. The enzyme substrate (H2O2) and the TMB-substrate (TMB) are added. After an appropriate time has elapsed for maximum colour development, the enzyme reaction is stopped and the absorbance is determined. Dehydroepiandrosterone Sulphate concentration in the sample is calculated based on a series by a set of standard. The colour intensity is inversely proportional to the dehydroepiandrosterone sulphate concentration in the sample.